(1) Sampling. Samples that are collected and analyzed must be representative of the biosolids that are applied to the land.

(2) Analytical methods.

(a) The most current version of the publications listed in this subsection are incorporated by reference. These publications are available for review during normal working hours at the Washington State Department of Ecology headquarters located at 300 Desmond Drive in Olympia, Washington. Copies may be obtained from the standard producer or publisher.

(b) Unless otherwise stipulated by the department, the following methods (or methods in 40 C.F.R. Part 136 or 40 C.F.R. Part 503) must be used to analyze samples of biosolids or sewage sludge.

ANALYTICAL METHODS

Parameter			Analytical Method
Arsenic			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7010
			SW-846 Method 7061
Cadmium			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Copper			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Lead			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Mercury			SW-846 Method 7470
			SW-846 Method 7471
Molybdenum			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Nickel			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Selenium			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7010
			SW-846 Method 7741
Zinc			SW-846 Method 6010
			SW-846 Method 6020
			SW-846 Method 7000B
			SW-846 Method 7010
Fecal Coliform			SM 9221 C or E
			SM 9222 D
			Appendix F, EPA/625/R-92/013
			EPA 1680
			EPA 1681
Salmonella Bacteria			SM 9260 D
			Appendix G, EPA/625/R-92/013
			EPA 1682
Helminth Ova			Appendix I, EPA/625/R-92/013
Enteric Viruses			ASTM Designation: D 4994-89
			Appendix H, EPA/625/R-92/013
Total Kjeldahl Nitrogen (TKN)			SM Method 4500, Norg B
			SM Method 4500, Norg C
Nitrate (as N)			SM Method 4500-NO3 E, F, or H
Nitrite (as N)			SM Method 4500-NO2 B
Ammonia (as N)			SM Method 4500-NH3 B + C, D, E, or G
Organic Nitrogen			Value calculated as TKN minus NH3-N
Total Phosphorus			SM Method 4500-P B + E or F
Total Solids, Fixed Solids, or Volatile Solids			SM Method 2540 G
Volatile Solids Reduction			Appendix C, EPA/625/R-92/013
Additional Volatile Solids Reduction for Anaerobically Digested Solids			Appendix D (1), EPA/625/R-92/013
Additional Volatile Solids Reduction for Aerobically Digested Solids			Appendix D (3), EPA/625/R-92/013
Specific Oxygen Update Rate			SM Method 2710 B
(SOUR)			Appendix D (2), EPA/625/R-92/013
pH			SW-846 Method 9045D
TCLP			SW-846 Method 1311
Paint Filter Test			SW-846 Method 9095B
Where:
ASTM	=	"Standard Practice for Recovery of Viruses From Wastewater Sludges," Annual Book of ASTM Standards: Section 11-Water and Environmental Technology, ASTM, 1916 Race Street, Philadelphia, PA 19103-1187.
EPA/625/R-92/013	=	"Environmental Regulations and Technology, Control of Pathogens and Vector Attraction in Sewage Sludge (Including Domestic Septage) Under 40 C.F.R. Part 503,"
		U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory, Center for Environmental Research Information, Cincinnati, OH 45268.
EPA 1680	=	USEPA. Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using Lauryl-Tryptose Broth (LTB) and EC Medium. U.S. Environmental Protection Agency, Office of Water, Washington, DC EPA-821-R-06-012.
EPA 1681	=	USEPA. Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 Medium. U.S. Environmental Protection Agency, Office of Water, Washington, DC EPA-821-R-06-013.
EPA 1682	=	USEPA. Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. U.S. Environmental Protection Agency, Office of Water, Washington, DC EPA-821-R-06-014.
SM	=	"Standard Methods for the Examination of Water and Wastewater," American Public Health Association, 1015 15th Street NW, Washington, DC 20005.
SW-846	=	"Test Methods for Evaluating Solid Waste, Physical/Chemical Methods," EPA publication SW-846. Available from the National Technical Information Service, 5285 Port Royal Road, Springfield, VA 22161.

PDFWAC 173-308-140

Biosolids sampling and analytical methods.